Real-time PCR has become a widely used technique for a variety of applications, such as gene expression, mutational analysis, and pathogen detection. Many biotechnology companies offer …

From Ct analysis of your real-time PCR gene expression experiments, to allele cluster analysis of Applied Biosystems™ TaqMan® SNP Assay data, to CNV copy number determination, to …

You have just finished your real-time quantitative reverse transcription PCR or RT-qPCR experiment. Now what? How do you analyze your data? To analyze your qPCR data, you will …

Based on the standard curve method and other useful data analysis methods, we present and compare four statistical approaches and models for the analysis of real-time PCR data.

Jul 2, 2025 · Analyzing RT-PCR data accurately is crucial for deriving meaningful biological insights. This article delves into the essential steps and considerations for analyzing RT-PCR …

real-time PCR include: PCR theoretically amplifies DNA exponentially, doubling the number of target molecules with ea. h amplification cycle. When it was first developed, scientists …

In Sections 5–7, we present sample protocols and data that demonstrate the use of real-time PCR in specific applications, namely, gene expression analysis, allelic discrimination, and …

ExpressionSuite Analysis Software can be found on our FGCLims system. Go to the LIMS login screen. The link to the download page is located on the PT-PCR page. For questions …

This guide provides an introduction to many of the technical aspects of real-time PCR. It includes guidelines for designing the best real-time PCR assay for your experiments and explains how …

Whether you're new to real-time PCR, also called quantitative PCR (qPCR), or want to learn about new applications for real-time PCR, we have the learning material, including videos and …